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KMID : 0382420020280020081
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Korean Journal of Environmental Health Society
2002 Volume.28 No. 2 p.81 ~ p.88
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Effect of Cyclohexanone Treatment on the Activities of Oxygen Free Radical Metabolizing Enzyme in the Liver
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Kim Hyun-Hee
Yoon Chong-Guk
Joh Hyun-Sung
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Abstract
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Effect of cyclohexanone treatment on the activities oxygen free radical and cyclohexanone metabolizing enzyme in acute liver damaged rats. was investigated. Acute liver damage was induced in rats with pretreatment of 50% CCl₄ in olive oil(O.1ml/l00g body wt) intraperitoneally 3 times every other day. CyclohexanoneO.56g/kg body wt. i.p,) was administered to the animals 24 hours after the last pretreatment of CCI₄. Rats were sacrificed at 4 hours after injection of cyclohexanone.
On the basis of liver weight/body weight(%), serum levels of alanine aminotransferase activity and hepatic protein content. cyclohexanone treatment to acute liver damaged animals led to the more enhanced liver damage.
On the other hand. injection of cyclohexanone to the rats led to the increased activities of hepatic cytochrome P-450 dependent aniline hydroxylase and xanthine oxidase. Furthermore. by treatment of cyclohexanone to the acute liver damaged rats hepatic xanthine oxidase activity was more increased than the CCI₄ treated rats. In case of oxygen free radical scavenging system, the hepatic glutathione content and the activities of hepatic glutathione S-transferase, catalase. superoxide dismutase were generally increased by injection of cyclohexanone to rats. and the hepatic glutathione content. catalase and alcohol dehydrogenase activities were more decreased in liver damaged rats by the treatment of cyclohexanone.
In conclusion, the cyclohexanone treatment to acute liver damaged rats led to enhancement of liver damage that may be due to oxygen free radical together with cyclohexanone.
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